First, we must understand what makes a student proficient in this area. A student is proficient if they: demonstrate the ability to prepare an agarose gel, calculates and measures volumes and masses correctly, boils agarose solution until no transparent particles are visible, the volume after heating is close to the original volume, they cool the molten agarose to around 55°C before pouring, places the comb in the right position, the agarose does not leak from the gel tray, the set gel has a smooth surface with no uniform texture, is 0.5-1.0 cm in-depth, and they can remove the comb once the gel is opaque.
Now that we know what makes a student proficient, we can begin to talk about where I have applied these skills. In the charge of the gel brigade lab, we practiced these skills before applying them in labs such as restriction digestion/analysis of lambda DNA and forensic DNA fingerprinting.
A Time Lapse of a Gel Electrophoresis Run
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